plko 1 gfp (Addgene inc)
95
Structured Review
Addgene inc
plko 1 gfp
Plko 1 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+gfp/pLKO%2E1+GFP+shRNA+(Plasmid+%2330323)/pm41811993-125-5-16
Average 95 stars, based on 173 article reviews
Plko 1 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+gfp/pLKO%2E1+GFP+shRNA+(Plasmid+%2330323)/pm41811993-125-5-16
Average 95 stars, based on 173 article reviews
plko 1 gfp - by Bioz Stars,
2026-09
95/100 stars
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Cloning:Article Title: FAM81A is a postsynaptic protein that regulates the condensation of postsynaptic proteins via liquid–liquid phase separation Article Snippet: To construct shRNA plasmids, shRNA sequences were designed using Invitrogen Block-iT RNAi Designer ( https://rnaidesigner.thermofisher.com/rnaiexpress/ ). .. The oligonucleotides were inserted into pLKO.1 - TRC cloning vector and Article Title: FAM81A is a postsynaptic protein that regulates the condensation of postsynaptic proteins via liquid-liquid phase separation. Article Snippet: To construct shRNA plasmids, shRNA sequences were designed using Invitrogen Block-iT RNAi Designer (https://rnaidesigner. thermofisher.com/rnaiexpress/). .. The oligonucleotides were inserted into pLKO.1 - TRC cloning vector and Article Title: DRACC1, a major postsynaptic protein, regulates the condensation of postsynaptic proteins via liquid-liquid phase separation Article Snippet: To construct shRNA plasmids, shRNA sequences were designed using Invitrogen Block-iT RNAi Designer ( https://rnaidesigner.thermofisher.com/rnaiexpress/ ). .. The oligonucleotides were inserted into pLKO.1 - TRC cloning vector and Plasmid Preparation:Article Title: FAM81A is a postsynaptic protein that regulates the condensation of postsynaptic proteins via liquid–liquid phase separation Article Snippet: To construct shRNA plasmids, shRNA sequences were designed using Invitrogen Block-iT RNAi Designer ( https://rnaidesigner.thermofisher.com/rnaiexpress/ ). .. The oligonucleotides were inserted into pLKO.1 - TRC cloning vector and Article Title: FAM81A is a postsynaptic protein that regulates the condensation of postsynaptic proteins via liquid-liquid phase separation. Article Snippet: To construct shRNA plasmids, shRNA sequences were designed using Invitrogen Block-iT RNAi Designer (https://rnaidesigner. thermofisher.com/rnaiexpress/). .. The oligonucleotides were inserted into pLKO.1 - TRC cloning vector and Article Title: OXSR1 inhibits inflammasome activation by limiting potassium efflux during mycobacterial infection Article Snippet: .. On the day of transfection, cells were co-transfected with 15 μg of the Article Title: DRACC1, a major postsynaptic protein, regulates the condensation of postsynaptic proteins via liquid-liquid phase separation Article Snippet: To construct shRNA plasmids, shRNA sequences were designed using Invitrogen Block-iT RNAi Designer ( https://rnaidesigner.thermofisher.com/rnaiexpress/ ). .. The oligonucleotides were inserted into pLKO.1 - TRC cloning vector and Transfection:Article Title: OXSR1 inhibits inflammasome activation by limiting potassium efflux during mycobacterial infection Article Snippet: .. On the day of transfection, cells were co-transfected with 15 μg of the Expressing:Article Title: OXSR1 inhibits inflammasome activation by limiting potassium efflux during mycobacterial infection Article Snippet: .. On the day of transfection, cells were co-transfected with 15 μg of the Clone Assay:Article Title: Aberrant splicing of MBD1 reshapes the epigenome to drive convergent myeloerythroid defects in MDS Article Snippet: .. C-terminal FLAG or V5 tagged MBD1 isoform open-reading frames, as well as the native BCOR open reading frame, were lentivirally overexpressed under the SFFV promoter using the pSMALB backbone, a gift from Drs. John Dick & Peter van Galen (Addgene ##161786, RRID: Addgene_161786). shRNAs were lentivirally expressed using Article Title: A-kinase anchoring protein 17 A promotes hypoxic adaptation and tumor growth by augmenting HIF1α protein synthesis. Article Snippet: Hypoxia-inducible factor 1α (HIF1α) is the master transcriptional regulator of cellular adaptation to low oxygen microenvironment, essential for oxygen homeostasis and promoting tumorigenesis.. We report that AKAP17A, an A-kinase anchoring protein, activates HIF1α signaling through a protein kinase A (PKA)-independent mechanism.. Depletion of AKAP17A in mammalian cells reduced HIF1α abundance and attenuated the transcriptional activation of HIF target genes. shRNA:Article Title: Aberrant splicing of MBD1 reshapes the epigenome to drive convergent myeloerythroid defects in MDS Article Snippet: .. C-terminal FLAG or V5 tagged MBD1 isoform open-reading frames, as well as the native BCOR open reading frame, were lentivirally overexpressed under the SFFV promoter using the pSMALB backbone, a gift from Drs. John Dick & Peter van Galen (Addgene ##161786, RRID: Addgene_161786). shRNAs were lentivirally expressed using Real-time Polymerase Chain Reaction:Article Title: Aberrant splicing of MBD1 reshapes the epigenome to drive convergent myeloerythroid defects in MDS Article Snippet: .. C-terminal FLAG or V5 tagged MBD1 isoform open-reading frames, as well as the native BCOR open reading frame, were lentivirally overexpressed under the SFFV promoter using the pSMALB backbone, a gift from Drs. John Dick & Peter van Galen (Addgene ##161786, RRID: Addgene_161786). shRNAs were lentivirally expressed using Generated:Article Title: A-kinase anchoring protein 17 A promotes hypoxic adaptation and tumor growth by augmenting HIF1α protein synthesis. Article Snippet: Hypoxia-inducible factor 1α (HIF1α) is the master transcriptional regulator of cellular adaptation to low oxygen microenvironment, essential for oxygen homeostasis and promoting tumorigenesis.. We report that AKAP17A, an A-kinase anchoring protein, activates HIF1α signaling through a protein kinase A (PKA)-independent mechanism.. Depletion of AKAP17A in mammalian cells reduced HIF1α abundance and attenuated the transcriptional activation of HIF target genes. Polymerase Chain Reaction:Article Title: A-kinase anchoring protein 17 A promotes hypoxic adaptation and tumor growth by augmenting HIF1α protein synthesis. Article Snippet: Hypoxia-inducible factor 1α (HIF1α) is the master transcriptional regulator of cellular adaptation to low oxygen microenvironment, essential for oxygen homeostasis and promoting tumorigenesis.. We report that AKAP17A, an A-kinase anchoring protein, activates HIF1α signaling through a protein kinase A (PKA)-independent mechanism.. Depletion of AKAP17A in mammalian cells reduced HIF1α abundance and attenuated the transcriptional activation of HIF target genes. Subcloning:Article Title: A-kinase anchoring protein 17 A promotes hypoxic adaptation and tumor growth by augmenting HIF1α protein synthesis. Article Snippet: Hypoxia-inducible factor 1α (HIF1α) is the master transcriptional regulator of cellular adaptation to low oxygen microenvironment, essential for oxygen homeostasis and promoting tumorigenesis.. We report that AKAP17A, an A-kinase anchoring protein, activates HIF1α signaling through a protein kinase A (PKA)-independent mechanism.. Depletion of AKAP17A in mammalian cells reduced HIF1α abundance and attenuated the transcriptional activation of HIF target genes. |