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plko 1 gfp  (Addgene inc)


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    Structured Review

    Addgene inc plko 1 gfp
    Plko 1 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+gfp/pLKO%2E1+GFP+shRNA+(Plasmid+%2330323)/pm41811993-125-5-16
    Average 95 stars, based on 173 article reviews
    plko 1 gfp - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cloning:

    Article Title: FAM81A is a postsynaptic protein that regulates the condensation of postsynaptic proteins via liquid–liquid phase separation
    Article Snippet: To construct shRNA plasmids, shRNA sequences were designed using Invitrogen Block-iT RNAi Designer ( https://rnaidesigner.thermofisher.com/rnaiexpress/ ). .. The oligonucleotides were inserted into pLKO.1 - TRC cloning vector and pLKO.1-GFP, according to the instruction ( http://www.addgene.org/protocols/plko/ ). ..

    Article Title: FAM81A is a postsynaptic protein that regulates the condensation of postsynaptic proteins via liquid-liquid phase separation.
    Article Snippet: To construct shRNA plasmids, shRNA sequences were designed using Invitrogen Block-iT RNAi Designer (https://rnaidesigner. thermofisher.com/rnaiexpress/). .. The oligonucleotides were inserted into pLKO.1 - TRC cloning vector and pLKO.1-GFP, according to the instruction (http://www.addgene.org/protocols/ plko/). ..

    Article Title: DRACC1, a major postsynaptic protein, regulates the condensation of postsynaptic proteins via liquid-liquid phase separation
    Article Snippet: To construct shRNA plasmids, shRNA sequences were designed using Invitrogen Block-iT RNAi Designer ( https://rnaidesigner.thermofisher.com/rnaiexpress/ ). .. The oligonucleotides were inserted into pLKO.1 - TRC cloning vector and pLKO.1-GFP, according to the instruction ( http://www.addgene.org/protocols/plko/ ). ..

    Plasmid Preparation:

    Article Title: FAM81A is a postsynaptic protein that regulates the condensation of postsynaptic proteins via liquid–liquid phase separation
    Article Snippet: To construct shRNA plasmids, shRNA sequences were designed using Invitrogen Block-iT RNAi Designer ( https://rnaidesigner.thermofisher.com/rnaiexpress/ ). .. The oligonucleotides were inserted into pLKO.1 - TRC cloning vector and pLKO.1-GFP, according to the instruction ( http://www.addgene.org/protocols/plko/ ). ..

    Article Title: FAM81A is a postsynaptic protein that regulates the condensation of postsynaptic proteins via liquid-liquid phase separation.
    Article Snippet: To construct shRNA plasmids, shRNA sequences were designed using Invitrogen Block-iT RNAi Designer (https://rnaidesigner. thermofisher.com/rnaiexpress/). .. The oligonucleotides were inserted into pLKO.1 - TRC cloning vector and pLKO.1-GFP, according to the instruction (http://www.addgene.org/protocols/ plko/). ..

    Article Title: OXSR1 inhibits inflammasome activation by limiting potassium efflux during mycobacterial infection
    Article Snippet: .. On the day of transfection, cells were co-transfected with 15 μg of the pLKO.1_GFP (#30323; Addgene) vector containing OXSR1_Sh1, OXSR1_Sh2 or AthmiR, 6.5 μg of the packaging plasmid pMDL-g/prre (#12251; Addgene), 2.5 μg of the packaging plasmid pRSV-Rev (#12253; Addgene), and 3.5 μg of the envelop expressing plasmid pMD2-VSV-G (#12259; Addgene) by the calcium phosphate transfection method. ..

    Article Title: DRACC1, a major postsynaptic protein, regulates the condensation of postsynaptic proteins via liquid-liquid phase separation
    Article Snippet: To construct shRNA plasmids, shRNA sequences were designed using Invitrogen Block-iT RNAi Designer ( https://rnaidesigner.thermofisher.com/rnaiexpress/ ). .. The oligonucleotides were inserted into pLKO.1 - TRC cloning vector and pLKO.1-GFP, according to the instruction ( http://www.addgene.org/protocols/plko/ ). ..

    Transfection:

    Article Title: OXSR1 inhibits inflammasome activation by limiting potassium efflux during mycobacterial infection
    Article Snippet: .. On the day of transfection, cells were co-transfected with 15 μg of the pLKO.1_GFP (#30323; Addgene) vector containing OXSR1_Sh1, OXSR1_Sh2 or AthmiR, 6.5 μg of the packaging plasmid pMDL-g/prre (#12251; Addgene), 2.5 μg of the packaging plasmid pRSV-Rev (#12253; Addgene), and 3.5 μg of the envelop expressing plasmid pMD2-VSV-G (#12259; Addgene) by the calcium phosphate transfection method. ..

    Expressing:

    Article Title: OXSR1 inhibits inflammasome activation by limiting potassium efflux during mycobacterial infection
    Article Snippet: .. On the day of transfection, cells were co-transfected with 15 μg of the pLKO.1_GFP (#30323; Addgene) vector containing OXSR1_Sh1, OXSR1_Sh2 or AthmiR, 6.5 μg of the packaging plasmid pMDL-g/prre (#12251; Addgene), 2.5 μg of the packaging plasmid pRSV-Rev (#12253; Addgene), and 3.5 μg of the envelop expressing plasmid pMD2-VSV-G (#12259; Addgene) by the calcium phosphate transfection method. ..

    Clone Assay:

    Article Title: Aberrant splicing of MBD1 reshapes the epigenome to drive convergent myeloerythroid defects in MDS
    Article Snippet: .. C-terminal FLAG or V5 tagged MBD1 isoform open-reading frames, as well as the native BCOR open reading frame, were lentivirally overexpressed under the SFFV promoter using the pSMALB backbone, a gift from Drs. John Dick & Peter van Galen (Addgene ##161786, RRID: Addgene_161786). shRNAs were lentivirally expressed using pLKO.1-GFP, cloned in house from pLKO.1-puromycin by replacing the puromycin resistance cassette with eGFP. pLKO.1-puromycin was a gift from Dr. Bob Weinberg (Addgene ##8453). shRNAs were selected from the Broad TRC shRNA Library and validated by qPCR. .. The control shScramble random-mer sequence was derived from PLKO.1-shScramble (Sabatini Lab, Addgene ##1864).

    Article Title: A-kinase anchoring protein 17 A promotes hypoxic adaptation and tumor growth by augmenting HIF1α protein synthesis.
    Article Snippet: Hypoxia-inducible factor 1α (HIF1α) is the master transcriptional regulator of cellular adaptation to low oxygen microenvironment, essential for oxygen homeostasis and promoting tumorigenesis.. We report that AKAP17A, an A-kinase anchoring protein, activates HIF1α signaling through a protein kinase A (PKA)-independent mechanism.. Depletion of AKAP17A in mammalian cells reduced HIF1α abundance and attenuated the transcriptional activation of HIF target genes.

    shRNA:

    Article Title: Aberrant splicing of MBD1 reshapes the epigenome to drive convergent myeloerythroid defects in MDS
    Article Snippet: .. C-terminal FLAG or V5 tagged MBD1 isoform open-reading frames, as well as the native BCOR open reading frame, were lentivirally overexpressed under the SFFV promoter using the pSMALB backbone, a gift from Drs. John Dick & Peter van Galen (Addgene ##161786, RRID: Addgene_161786). shRNAs were lentivirally expressed using pLKO.1-GFP, cloned in house from pLKO.1-puromycin by replacing the puromycin resistance cassette with eGFP. pLKO.1-puromycin was a gift from Dr. Bob Weinberg (Addgene ##8453). shRNAs were selected from the Broad TRC shRNA Library and validated by qPCR. .. The control shScramble random-mer sequence was derived from PLKO.1-shScramble (Sabatini Lab, Addgene ##1864).

    Real-time Polymerase Chain Reaction:

    Article Title: Aberrant splicing of MBD1 reshapes the epigenome to drive convergent myeloerythroid defects in MDS
    Article Snippet: .. C-terminal FLAG or V5 tagged MBD1 isoform open-reading frames, as well as the native BCOR open reading frame, were lentivirally overexpressed under the SFFV promoter using the pSMALB backbone, a gift from Drs. John Dick & Peter van Galen (Addgene ##161786, RRID: Addgene_161786). shRNAs were lentivirally expressed using pLKO.1-GFP, cloned in house from pLKO.1-puromycin by replacing the puromycin resistance cassette with eGFP. pLKO.1-puromycin was a gift from Dr. Bob Weinberg (Addgene ##8453). shRNAs were selected from the Broad TRC shRNA Library and validated by qPCR. .. The control shScramble random-mer sequence was derived from PLKO.1-shScramble (Sabatini Lab, Addgene ##1864).

    Generated:

    Article Title: A-kinase anchoring protein 17 A promotes hypoxic adaptation and tumor growth by augmenting HIF1α protein synthesis.
    Article Snippet: Hypoxia-inducible factor 1α (HIF1α) is the master transcriptional regulator of cellular adaptation to low oxygen microenvironment, essential for oxygen homeostasis and promoting tumorigenesis.. We report that AKAP17A, an A-kinase anchoring protein, activates HIF1α signaling through a protein kinase A (PKA)-independent mechanism.. Depletion of AKAP17A in mammalian cells reduced HIF1α abundance and attenuated the transcriptional activation of HIF target genes.

    Polymerase Chain Reaction:

    Article Title: A-kinase anchoring protein 17 A promotes hypoxic adaptation and tumor growth by augmenting HIF1α protein synthesis.
    Article Snippet: Hypoxia-inducible factor 1α (HIF1α) is the master transcriptional regulator of cellular adaptation to low oxygen microenvironment, essential for oxygen homeostasis and promoting tumorigenesis.. We report that AKAP17A, an A-kinase anchoring protein, activates HIF1α signaling through a protein kinase A (PKA)-independent mechanism.. Depletion of AKAP17A in mammalian cells reduced HIF1α abundance and attenuated the transcriptional activation of HIF target genes.

    Subcloning:

    Article Title: A-kinase anchoring protein 17 A promotes hypoxic adaptation and tumor growth by augmenting HIF1α protein synthesis.
    Article Snippet: Hypoxia-inducible factor 1α (HIF1α) is the master transcriptional regulator of cellular adaptation to low oxygen microenvironment, essential for oxygen homeostasis and promoting tumorigenesis.. We report that AKAP17A, an A-kinase anchoring protein, activates HIF1α signaling through a protein kinase A (PKA)-independent mechanism.. Depletion of AKAP17A in mammalian cells reduced HIF1α abundance and attenuated the transcriptional activation of HIF target genes.



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